Cell Culture:
Article Title: Cpne3 Regulates the Cell Proliferation and Apoptosis in Human Gbm via the Activation of Pi3k/akt Signaling Pathway
Article Snippet: .. Methods And Materials Cell lines and cell culture Human glioma cell lines (A172, T98G, U251 and U87), human glial cell line HEB and human HEK293T cell line were obtained from American Type Culture Collection (ATCC, USA). .. A172, T98G, U251, U87, HEB, HEK293T cells were cultured in DMEM (Hyclone, USA) supplemented with 10% heat-inactive FBS, 100 U/mL penicillin and 100 μg/mL streptomycin.
Article Title: Enhanced Glycolysis Confers Resistance Against Photon but Not Carbon Ion Irradiation in Human Glioma Cell Lines
Article Snippet: Bovine serum albumin (cat# B2064-100G) of Australian origin was purchased from Sigma-Aldrich, Burlington, MA, USA. .. Cell Culture Human glioma cell lines (U87 (ATCC # HTB-14), and LN229 (ATCC # CRL-2611)) were purchased from the American Type Culture Collection, while the U251 cell line was purchased from Procell (Wuhan, China). ..
Article Title: <p>Toosendanin Suppresses Glioma Progression Property and Induces Apoptosis by Regulating miR-608/Notch Axis</p>
Article Snippet: .. Cell Culture Human glioma cell lines (U-138MG and U-251MG) were all obtained from American Type Culture Collection (ATCC, Rockville, MD). .. Human normal astrocytes (NHA) were obtained from ScienCell (San Diego, CA), and served as a normal counterpart of glioma.
Article Title: Alternative magnetic stimulating system for preventing or treating brain tumor growth
Article Snippet: .. 1-1: Cell Culture Human glioma cell lines (U87MG (ATCC® HTB-14TM) and LN18 (ATCC® CRL-2610TM)) and the mouse brain endothelial cell line (bEnd3 (ATCC CRL2299TM)), purchased from the ATCC, were cultured in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% FBS (fetal bovine serum) and 100 U/ml penicillin-streptomycin under the conditions of 37° C. and 5% CO2. .. 1-2: Primary Culture of Neurons and Astrocytes Cortex was separated from the brains of 1 to 3-day-old mouse (C57BL/6J(B6) male and female mice, Jackson), and then maintained in 0.25% trypsin (Gibco, GIB-25200-072) solution (HBSS solution+6% trypsin+2% L-cysteine) at 37° C. for 20 minutes and separated into individual cells by pipetting.
Article Title: Molecular mechanism of m6A methylation of circDCL1 mediated by RNA methyltransferase METTL3 in the malignant proliferation of glioma cells
Article Snippet: .. Cell culture Human glioma cell lines (T98G, LN229, A172, and LN18) and healthy glioma cell line (HEB) were obtained from American Type Culture Collection (Manassas, Virginia, USA) and cultured in RPMI-1640 medium (HyClone, South Logan, UT, USA) containing 10% heat-inactivated fetal bovine serum (Invitrogen, Page 4/27 Carlsbad, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scienti c, Waltham, MA, USA) at 37°C with 5% CO2. .. Cell treatment Three siRNAs speci cally targeting circDCL1, circDCL1 pcDNA 3.1, METTL3 pcDNA 3.1, three siRNAs speci cally targeting catenin beta interacting protein 1 (CTNNBIP1), and their negative controls (NCs) were designed and synthesized by GenePharma (Shanghai, China).
Article Title: Small‐molecule inhibition of prostaglandin E receptor 2 impairs cyclooxygenase‐associated malignant glioma growth
Article Snippet: .. Cell culture Human glioma cell lines—LN229 (ATCC Cat # CRL‐2611, RRID:CVCL_0393) and SF767 (RRID:CVCL_6950)—and the COX‐2‐overexpressing cells derived from these lines were generously provided by Dr. Kaiming Xu and Dr. Hui‐Kuo G. Shu from the Winship Cancer Institute of Emory University and authenticated by genetic profiling using polymorphic short tandem repeat loci (ATCC, 2011 ; Xu et al., 2014 ). .. The cells were cultured in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% (v:v) FBS (HyClone, Pittsburgh, PA, USA) and penicillin (100 U·ml −1 )/streptomycin (100 μg·ml −1 ; Gibco) in a humidified incubator at 37°C with 5% CO 2 .
Modification:Article Title: Alternative magnetic stimulating system for preventing or treating brain tumor growth
Article Snippet: .. 1-1: Cell Culture Human glioma cell lines (U87MG (ATCC® HTB-14TM) and LN18 (ATCC® CRL-2610TM)) and the mouse brain endothelial cell line (bEnd3 (ATCC CRL2299TM)), purchased from the ATCC, were cultured in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% FBS (fetal bovine serum) and 100 U/ml penicillin-streptomycin under the conditions of 37° C. and 5% CO2. .. 1-2: Primary Culture of Neurons and Astrocytes Cortex was separated from the brains of 1 to 3-day-old mouse (C57BL/6J(B6) male and female mice, Jackson), and then maintained in 0.25% trypsin (Gibco, GIB-25200-072) solution (HBSS solution+6% trypsin+2% L-cysteine) at 37° C. for 20 minutes and separated into individual cells by pipetting.
Derivative Assay:Article Title: Small‐molecule inhibition of prostaglandin E receptor 2 impairs cyclooxygenase‐associated malignant glioma growth
Article Snippet: .. Cell culture Human glioma cell lines—LN229 (ATCC Cat # CRL‐2611, RRID:CVCL_0393) and SF767 (RRID:CVCL_6950)—and the COX‐2‐overexpressing cells derived from these lines were generously provided by Dr. Kaiming Xu and Dr. Hui‐Kuo G. Shu from the Winship Cancer Institute of Emory University and authenticated by genetic profiling using polymorphic short tandem repeat loci (ATCC, 2011 ; Xu et al., 2014 ). .. The cells were cultured in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% (v:v) FBS (HyClone, Pittsburgh, PA, USA) and penicillin (100 U·ml −1 )/streptomycin (100 μg·ml −1 ; Gibco) in a humidified incubator at 37°C with 5% CO 2 .
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